SL-PAb002 / ALLERGEN RESEARCH

Der p 2Mouse polyclonal antibody

For detecting and analysing the house dust mite allergen Der p 2 in research applications.

Download product datasheet (PDF)

For research use only. Not for diagnostic or therapeutic use.

01 / OVERVIEW

An antibody for
Der p 2 research

SL-PAb002 is a mouse polyclonal antibody raised against recombinant Der p 2. It is used in Western blot (WB) analysis of recombinant Der p 2 protein and Dermatophagoides pteronyssinus extract for allergen research.

The extract experiment uses Dermatophagoides pteronyssinus. Suitability for other mite species or allergens requires independent validation.

Evaluate ELISA suitability and performance with the intended samples and experimental conditions.

02 / EXPERIMENTAL RESULTS

Western blot
results

Western blot results for recombinant proteins and extracts. Open an image for a closer view.

WB / 01

Recombinant Der p 2 and mite extract

SL-PAb002 is used to detect recombinant Der p 2 and Dermatophagoides pteronyssinus extract (Dp extract) by Western blot. Sample identities and loading amounts are shown in the figure and caption.

Result interpretation and scope

Sample composition and loading amounts differ. This WB image does not quantify antigen abundance, affinity or a detection limit.

SL-PAb002 Western blot with molecular weight markers, 625 ng recombinant Der p 2 and 50 µg Dermatophagoides pteronyssinus extract.Enlarge image
Figure 1 | Recombinant Der p 2: 625 ng. Dp extract: 50 µg. Molecular weight markers and sample lanes are labelled in the image.

Result scope: these WB results relate to the samples and conditions shown. Other samples and applications require independent validation. Images do not quantify affinity, purity or clinical effects.

Discuss assay suitability & collaboration

03 / SPECIFICATIONS

Specifications
& conditions

Use the recommended dilutions as starting points for optimization. Select working conditions for your samples and assay setup.

Catalog number
SL-PAb002
Antibody type
Mouse polyclonal antibody
Immunogen
Recombinant Der p 2 allergen protein
Immunogen GenBank accession
AAF86462.1
Reactive species
Dermatophagoides pteronyssinus
Applications
ELISA, Western blot (WB)
Recommended dilution
1:1000–1:5000 (ELISA / WB; optimize for the specific experiment)
Formulation
PBS with 1% BSA; preservative-free
Storage
−20°C to −80°C; avoid repeated freeze–thaw cycles
Storage stability
12 months from the date of receipt under the stated storage conditions
Intended use
For research use only. Not for diagnostic or therapeutic use.

04 / APPLICATIONS & USE

Application & handling guidance

Application suitability

Plan research applications using ELISA or WB, with appropriate validation for each method and sample type.

Optimize for the experiment

Use the recommended 1:1000–1:5000 dilution range as a starting point, then evaluate suitability with the intended samples, assay setup and controls.

Storage and research use

Store at −20°C to −80°C and avoid repeated freeze–thaw cycles. Under these conditions, the storage stability is 12 months from receipt. For research use only, not diagnosis or treatment.

05 / COLLABORATION

Discuss antibody applications
& collaboration

Tell us your research goal, sample types and intended methods. For bulk or distribution enquiries, include your estimated requirements and collaboration interests.

  • Research evaluation and application suitability
  • Experimental validation and assay optimization
  • Bulk requirements and distribution discussions

Feasibility, materials and collaboration terms are discussed for the individual project.

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